Altered populations of acidic chromatin proteins in breast cancer cells.

The acidic chromatin proteins extracted from normal and neoplastic mammary cells of C3H mice and Fischer rats have been compared by polyacrylamide gel electrophoresis, amino acid analysis, and radioactivity labeling patterns of synthesis in vitro. Following purification of chromatin from purified nuclei and extraction of histones in 2 M sodium chloride, the acidic proteins associated with DNA were subjected to electrophore sis on polyacrylamide gels containing sodium dodecyl sulfate, 'and the stained gels were compared by absorbance scanning.